Gain of Fiction

virus, infectious agent of small size and simple composition that can multiply only in living cells of animals, plants, or bacteria. The name is from a Latin word meaning “slimy liquid” or “poison.” https://www.britannica.com/science/virus I have purposefully stayed away from the whole “SARS-COV-2” as a gain of function/bioweapon disinformation campaign as it is obvious to anyone who has ever read… Continue reading Gain of Fiction

The Case Against “Viral” Genomes

I’ve come across quite a few people who seem to believe that the existence of “viral” genomes and “viral’ RNA or DNA sequences somehow proves the existence of “viruses.” One person even believed that the genome was a representation of purified/isolated “viruses.” Disregarding the fact that random A,C,T,G’s that exist only inside a computer database… Continue reading The Case Against “Viral” Genomes

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Limitations in Genome Sequencing Technology and Data Analysis

When you understand that the process used to obtain a “viral” genome involves numerous complex steps each with their own ability to introduce biases, errors, artefacts, etc which can easily propagate into the final result, it becomes clear to see how the accuracy and reliability of a “viral” genome can be questioned. There are too… Continue reading Limitations in Genome Sequencing Technology and Data Analysis

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The Challenges in Genome Library Construction

What is truly revolutionary about molecular biology in the post-Watson-Crick era is that it has become digital…the machine code of the genes is uncannily computer-like.” -Richard Dawkins According to the Britannica, a genomic library is essentially “a collection of DNA fragments that make up the full-length genome of an organism. A genomic library is created… Continue reading The Challenges in Genome Library Construction

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The Hypothetical Conversion of RNA to cDNA

In order to create a “viral” genome, there are many steps and different processes that the sample must go through first to be prepared for sequencing. Normally the sample is subjected to toxic cell culturing in order to grow enough “virus” to utilize the unpurified culture supernatant for sequencing. Sometimes the sequencing is done straight… Continue reading The Hypothetical Conversion of RNA to cDNA

DNA and RNA Shearing/Fragmentation

There are numerous steps that go into the creation of a genome, whether human, animal, bacterial, or “viral.” They are often complex and require various methods and technologies such as PCR amplification and sequencers in order to obtain the final product. As the process is a very intricate and complicated one, there are multiple ways… Continue reading DNA and RNA Shearing/Fragmentation

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The Challenges Related to RNA Extraction for Genome Sequencing

The critical first step to generate a genome after the cell culturing process is to extract the RNA from the mixture. The purpose of this is to break down and isolate RNA from any other cellular components and impurities that are within the culture supernatant. It is important to understand that through this process, they… Continue reading The Challenges Related to RNA Extraction for Genome Sequencing

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Genome Contamination: A Widespread Problem

Most clinical specimens and tissue culture samples to be used for viral genome sequencing are usually contaminated with human cells, other microorganisms and naked DNA and RNA from disrupted cells. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2638583/ When talking about the proof for the existence of “viruses,” it is logical to require that the particles believed to be a “virus” are… Continue reading Genome Contamination: A Widespread Problem

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Categorized as Genomics