Passage in Vero Cells: The Variant Game

“In addition, passage in cell culture can result in artificial mutations in the sequences, which were not present in the original clinical sample. This can have major implications for subsequent analyses. Using cell culture solely for the purpose of amplifying virus genetic material for SARS-CoV-2 sequencing should therefore be avoided“ https://www.google.com/url?sa=t&source=web&rct=j&url=https://apps.who.int/iris/rest/bitstreams/1326052/retrieve&ved=2ahUKEwi9i5ql-M7xAhUJnGoFHQvEBaIQFjADegQICBAC&usg=AOvVaw0gjHudaoThq3mU1JVxALpd The above statement comes… Continue reading Passage in Vero Cells: The Variant Game

Cell Line Misidentification

“Evidence suggests that up to one-third of tumor cell lines being used in scientific research are affected by inter- or intraspecies cross-contamination or have been wrongly identified, thereby rendering many of the conclusions doubtful if not completely invalid.” — Lancet Oncology, vol. 2, July 2001, p. 393 Cell line misidentification is a huge problem in… Continue reading Cell Line Misidentification