Isolation: the act of separating something from other things : the act of isolating something
Above is the definition most people agree with and refer to when isolating something. Not virologists. This is what they mean when using the word isolation:
“Viruses are basically inanimate objects which need a culture to activate in. But the way they are phrasing the requests is that the sample must be completely unadulterated and not be grown in any culture – and you can’t do that,” she told AAP FactCheck in a phone interview.
“You can’t isolate a virus without using a cell culture, so by using their definition it hasn’t been isolated. But it has been isolated and cultivated using a cell culture multiple times all around the world.”
The above quote is from a Facebook “factcheck.”
In layman’s terms, if using the agreed upon definition of isolation, virologists agree that they haven’t isolated a “virus” from everything else and admit that this logical requirement is impossible. But they rationalize that this is ok because it’s a “virus.” It is assumed (as this was never proven) that a “virus” needs a host cell (which should be from the host they take the “virus” from but try not to think too hard about that logical inconsistency) in order to grow and replicate. But not just any host cell will do. In the case of “SARS-COV-2,” it needs the kidney cells from an African Green Monkey.
But wait, there’s more!
It also needs to be immediately placed in “Viral” Transport Media after being taken from a patient. This normally consists of animal DNA, antibiotics, and other chemicals/nutrients. In order to grow, it needs fetal bovine serum (blood taken from the hearts of baby cows). In order to be free of bacteria, it needs 2 or 3 cell toxic antibiotics. In order to “eat,” it needs various unknown nutrients/chemicals in DMEM. All of this must be added to the unpurified sample (which in and of itself contains billions of particles) from a patient, mixed together, and then incubated for days.
Once the expected Cytopathogenic Effect (i.e. cell death) is seen in the petri dish, then and only then do virologists claim a “virus” been “isolated.”
Virology subscribes to subtraction through addition. Or, in other words, the exact opposite of isolation.
It is obvious no “virus” has ever been properly purified nor isolated. If you have to change the meaning of isolation in order to claim you isolated something, you never did.